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Prediction analyses were performed through backward stepwise Cox proportional hazards regression and expressed as the hazard ratio (HR)

Biotin has an extremely high affinity toward streptavidin (Kd 1014 nM), enabling cell lysis and affinity capture under harsh conditions and thus the near complete extraction of a cells proteome (Samavarchi-Tehrani et al., 2020)

Here are some of the main roles vitamin B12 plays in health maintenance: In addition to those numerous functions, vitamin B12 also offers critical immune system support

The supernatants were collected by centrifugation at 5000 rpm for 5 min at 4 C and precleared by incubated with GFP-Trap agarose beads (Chromotek) or HA-Beads (Roche) for 2 h at 4 C with rotation